Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR
نویسندگان
چکیده
منابع مشابه
Distribution of Enterococcal Species and Detection of Vancomycin Resistance Genes by Multiplex PCR in Tehran Sewage
Enterococci are important because of their role as the leading cause of nosocomial infections which have a significant role in the dissemination and persistence of antimicrobial resistance genes. Methods: In this study, we determined the distribution of enterococcal species in the sewage treatment plants in Iran. Furthermore, we improved a rapid and specific PCR method using primers (sodA and ...
متن کاملFood allergy population thresholds: DBPCFC data for rice, kiwi, apple, peach, carrot, maize, tomato, and other less common allergenic foods
The “Big 8” food allergens consists of cow’s milk, eggs, wheat, peanuts, tree nuts, soybeans, fish, and crustacean shellfish and account for ~90% of the allergic reactions to foods. During the scientific review of the VITAL (Voluntary Incidental Trace Allergen Labelling) program, individual challenge data were gathered for the Big 8 food allergens (plus mustard, sesame and celery) and, where po...
متن کاملSimultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR
A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in total RNA of pome trees extracted with a CTAB method. The sensitivity of the method was established using in vitro synthesized viral trans...
متن کاملSimultaneous Detection of Helicobacter Genus and Helicobacter pylori Species using a Multiplex PCR Method
In order to improve simultaneous detection and identification of Helicobacter genus in general and Helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex PCR. In this study, two pairs of primers: Hcom1 and Hcom2 specific for Helicobacter genus, Hicd1 and Hicd2 specific for Helicobacter pylori species were used. To determine the sensitivity of...
متن کاملAllergen Data Collection - Update: Kiwi Fruit (Actinidia chinensis)
First reports of allergic reactions to kiwi fruit occurred relatively late in 1981, when this exotic fruit was becoming more common in nutrition in western countries. The prevalence of kiwi fruit allergy seems to be low in food allergic individuals. Nevertheless IgE mediated reactions after ingestion of kiwi fruit can be most severe, as shown by several cases of anaphylactic shocks. Belonging t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Food Science and Biotechnology
سال: 2019
ISSN: 1226-7708,2092-6456
DOI: 10.1007/s10068-019-00591-y